2012 ha taz addgene Search Results


2012 ha taz addgene  (Addgene inc)
94
Addgene inc 2012 ha taz addgene
2012 Ha Taz Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcs bira r118g ha vector  (Addgene inc)
94
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pdnr201 dr junjie chen  (Addgene inc)
96
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gcamp5g  (Addgene inc)
93
Addgene inc gcamp5g
Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration <t>(GCaMP5G)</t> and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.
Gcamp5g, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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najdi et  (Addgene inc)
90
Addgene inc najdi et
Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration <t>(GCaMP5G)</t> and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.
Najdi Et, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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noboru mizushima  (Addgene inc)
93
Addgene inc noboru mizushima
Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration <t>(GCaMP5G)</t> and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.
Noboru Mizushima, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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expression constructs  (Addgene inc)
96
Addgene inc expression constructs
Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration <t>(GCaMP5G)</t> and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.
Expression Constructs, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mcherry chutr  (Addgene inc)
93
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Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration <t>(GCaMP5G)</t> and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.
Mcherry Chutr, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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myc destination dr junjie chen  (Addgene inc)
96
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Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration <t>(GCaMP5G)</t> and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.
Myc Destination Dr Junjie Chen, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phil sharp  (Addgene inc)
93
Addgene inc phil sharp
Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration <t>(GCaMP5G)</t> and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.
Phil Sharp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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r geco  (Addgene inc)
93
Addgene inc r geco
Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration <t>(GCaMP5G)</t> and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.
R Geco, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna3 1 mycbioid  (Addgene inc)
94
Addgene inc pcdna3 1 mycbioid
Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration <t>(GCaMP5G)</t> and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.
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Image Search Results


Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration (GCaMP5G) and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.

Journal: Journal of cell science

Article Title: Cortical mitochondria regulate insulin secretion by local Ca 2+ buffering in rodent beta cells.

doi: 10.1242/jcs.228544

Figure Lengend Snippet: Figure 1. Characterization and modulation of sub-plasma membrane mitochondria A. TIRF microscopy images of a MIN6 cell expressing mApple-Tomm20 before and during stimulation with 20 mM glucose. B. Quantification of the sub-plasma membrane density change following 15 min exposure 20 mM glucose (n=36 cells, P>0.1, paired 2-tailed Student’s t-test). C. TIRF microscopy recording of the cytosolic Ca2+ concentration (GCaMP5G) and cortical mitochondria (mApple-Tomm20) in a glucose-stimulated MIN6 cell.

Article Snippet: The following plasmids were used in this study: GFP-CRY2-OCRL (Idevall-Hagren et al., 2012), CIBN-GFP-CAAX (Idevall-Hagren et al., 2012), mApple-TOMM20-N1 (Addgene plasmid #54955), R-GECO (Addgene plasmid #32444 (Zhao et al., 2011)), GCaMP5G (Addgene plasmid #31788 (Akerboom et al., 2012)), mCherry-CHUtr (Addgene plasmid #26740 (Burkel et al., 2007)), mCherry- C1aC1bPKC (Wuttke et al., 2013), iRFP-PH-PLC1 (Idevall-Hagren et al., 2012), mito- LAR-GECO (Addgene plasmid 61245 (Wu et al., 2014)), 5HT6-G-GECO (Addgene plasmid 47499 (Su et al., 2013)).

Techniques: Clinical Proteomics, Membrane, Microscopy, Expressing, Concentration Assay

Figure 3. Cortical F-actin is modulated by Ca2+. A. Kymographs from TIRF microscopy recordings of INS-1E cells expressing GCaMP5G and mCh-CHUtr following depolarization in the absence or presence of Verapamil (100 µM). B. TIRF microscopy recordings of GCaMP5G and mCh-CHUtr fluorescence changes in response to the indicated stimuli. C. Quantifications of the area under the curve for the traces in (B) (Student’s unpaired t-test: nGCAMP5G=5, PGCAMP5G=0.0241; nmCh-CHUtr=6, PmCh-CHUtr=0.02).

Journal: Journal of cell science

Article Title: Cortical mitochondria regulate insulin secretion by local Ca 2+ buffering in rodent beta cells.

doi: 10.1242/jcs.228544

Figure Lengend Snippet: Figure 3. Cortical F-actin is modulated by Ca2+. A. Kymographs from TIRF microscopy recordings of INS-1E cells expressing GCaMP5G and mCh-CHUtr following depolarization in the absence or presence of Verapamil (100 µM). B. TIRF microscopy recordings of GCaMP5G and mCh-CHUtr fluorescence changes in response to the indicated stimuli. C. Quantifications of the area under the curve for the traces in (B) (Student’s unpaired t-test: nGCAMP5G=5, PGCAMP5G=0.0241; nmCh-CHUtr=6, PmCh-CHUtr=0.02).

Article Snippet: The following plasmids were used in this study: GFP-CRY2-OCRL (Idevall-Hagren et al., 2012), CIBN-GFP-CAAX (Idevall-Hagren et al., 2012), mApple-TOMM20-N1 (Addgene plasmid #54955), R-GECO (Addgene plasmid #32444 (Zhao et al., 2011)), GCaMP5G (Addgene plasmid #31788 (Akerboom et al., 2012)), mCherry-CHUtr (Addgene plasmid #26740 (Burkel et al., 2007)), mCherry- C1aC1bPKC (Wuttke et al., 2013), iRFP-PH-PLC1 (Idevall-Hagren et al., 2012), mito- LAR-GECO (Addgene plasmid 61245 (Wu et al., 2014)), 5HT6-G-GECO (Addgene plasmid 47499 (Su et al., 2013)).

Techniques: Microscopy, Expressing, Fluorescence